Idylla™’s sample-to-result functionality stems from the complete integration of all process steps, from sample liquefaction and cell lysis, over DNA/RNA extraction and real-time PCR amplification & detection, to data analysis and reporting.


1: Liquefaction

The physical properties of many clinical sample types make them unsuitable for fluid handling systems. For certain sample types, such as sputum or feces, the viscosity or consistency of the sample is too high while other sample types, such as fresh or formalin-fixed paraffin-embedded (FFPE) tissue, are completely solid. Before such sample types can be adequately processed by fluid-handling systems, they must be liquefied and homogenized.

2: Cell Lysis

The DNA or RNA that needs to be detected can be encapsulated (e.g. the bacterial/fungal cell wall or the viral membrane). Lysis is the process of liberating these target nucleic acids by disrupting the capsule in which they are contained. Certain bacterial and fungal cells have a particularly strong cell wall and disrupting them requires advanced lysis processes.

3: DNA/RNA Extraction

Most clinical sample types contain components that may interfere with the downstream target amplification and detection steps. Hence a nucleic acid extraction step is used to purify the nucleic acids from the sample. This extraction process can also serve as a way to increase the concentration of the target nucleic acids.


4: Real-time PCR amplification & detection

Idylla™’s high sensitivity allows detecting individual target molecules in a background of high amounts of other nucleic acid molecules. This sensitivity is achieved through real-time PCR, a powerful amplification technique. PCR allows a million or billion-fold multiplication of specific target molecules from a sample. Certain assays can detect a single nucleic acid target, while other multiplex assays can detect many different nucleic acid targets.

5: Data Analysis & Reporting

At the end of each test, Idylla™ automatically reports the results. Through bidirectional connectivity with laboratory and hospital information management systems, results can be forwarded with great accuracy and speed.